Intracellular Crystalline Ergosterol in Neurospora
نویسندگان
چکیده
In the fungus Neurospora crassa, hexagonal crystalline inclusions have been observed with both the light and electron microscopes. These crystals have been enriched by differential centrifugation and found to be identical with ergosterol by the criteria of ultraviolet spectral analysis and cytochemical analysis. Observations have been made on the distribution and fine structure of the crystalline bodies in various wild type and mutant strains of N. crassa.
منابع مشابه
Isotopic acetate and isovalerate in the synthesis of ergosterol by Neurospora.
The utilization of deuteroacetate for the synthesis of ergosterol in an acetateless Neurospora mutant has been described in an earlier paper (1). In this work it was demonstrated that at least 25 per cent of the hydrogen atoms of ergosterol is derived directly from acetate. Since the use of deuterium as a tracer necessarily involves errors of unknown magnitude due to the lability of deuterium a...
متن کاملSterol C-22 Desaturase ERG5 Mediates the Sensitivity to Antifungal Azoles in Neurospora crassa and Fusarium verticillioides
Antifungal azoles inhibit ergosterol biosynthesis by interfering with lanosterol 14α-demethylase. In this study, seven upregulated and four downregulated ergosterol biosynthesis genes in response to ketoconazole treatment were identified in Neurospora crassa. Azole sensitivity test of knockout mutants for six ketoconazole-upregulated genes in ergosterol biosynthesis revealed that deletion of on...
متن کاملDeletion of homologs of the SREPB pathway results in hyper-production of cellulases in Neurospora crassa and Trichoderma reesei
BACKGROUND The filamentous fungus Neurospora crassa efficiently utilizes plant biomass and is a model organism for genetic, molecular and cellular biology studies. Here, a set of 567 single-gene deletion strains was assessed for cellulolytic activity as compared to the wild-type parental strain. Mutant strains included were those carrying a deletion in: (1) genes encoding proteins homologous to...
متن کاملThe ergosterol biosynthesis pathway, transporter genes, and azole resistance in Aspergillus fumigatus.
The continuous use of triazoles can result in the development of drug resistance. Azole-resistant clinical isolates, spontaneous and induced mutants of Aspergillus fumigatus have been documented. The azoles block the ergosterol biosynthesis pathway by inhibiting the enzyme 14-alpha-demethylase, product of the CYP51. Fungal azole resistance involves both amino acid changes in the target site tha...
متن کاملAbnormal Ergosterol Biosynthesis Activates Transcriptional Responses to Antifungal Azoles
Fungi transcriptionally upregulate expression of azole efflux pumps and ergosterol biosynthesis pathway genes when exposed to antifungal agents that target ergosterol biosynthesis. To date, these transcriptional responses have been shown to be dependent on the presence of the azoles and/or depletion of ergosterol. Using an inducible promoter to regulate Neurospora crassa erg11, which encodes th...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Journal of Biophysical and Biochemical Cytology
دوره 11 شماره
صفحات -
تاریخ انتشار 1961